大麻二酚减轻甲基苯丙胺诱导的大鼠单胺类神经递质改变
作者:
作者单位:

昆明医科大学法医学院,国家卫生健康委员会毒品依赖和戒治重点实验室,昆明 650500

作者简介:

通讯作者:

中图分类号:

R-33

基金项目:


Cannabidiol attenuates methamphetamine-induced changes in monoamine neurotransmitters in rats
Author:
Affiliation:

School of Forensic Medicine, Key Laboratory of Drug Addiction Medicine of National Health Commission (NHC), Kunming Medical University, Kunming 650500, China

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 探索大麻二酚(cannabidiol, CBD)在甲基苯丙胺(methamphetamine, METH)依赖大鼠中对中枢神经系统单胺类神经递质的影响,阐释 CBD 对 METH 依赖的治疗机制,为毒品依赖戒治药物的研发提供参考。 方法 通过 METH(2 mg / kg)重复腹腔注射和条件位置偏爱(conditioned place preference, CPP)建立 METH 依赖大鼠模型;提取大鼠伏隔核(nucleus accumbens, NAc)、额叶皮质(frontal cortex, FC)、中脑腹侧被盖( ventral tegmental area, VTA)、纹状体(caudate putamen, CPu)和海马(hippocampus, Hip)等五个奖赏系统相关脑区,高效液相色谱法分离多巴胺(dopamine, DA)、五羟色胺(5-hydroxytryptamine, 5-HT)和去甲肾上腺素(norepinephrine, NE)三种单胺类神经递质;质谱分析定量三种单胺类神经递质水平。 结果 METH(2 mg / kg)重复给药可在大鼠 NAc、FC、VTA、CPu 和 Hip 中显著上调 DA 水平和下调 5-HT 水平,并伴随着 NAc 和 VTA 中 NE 水平上调;而 CBD(10、20、40、80mg / kg)前干预可剂量依赖性地削弱 METH 对上述单胺类神经递质的影响。 结论 CBD 可能通过维持大鼠奖赏脑区中单胺类神经递质的稳态水平,削弱 METH 依赖导致的奖赏效应。

    Abstract:

    Objective This work was aimed at investigating the intervention effects of cannabidiol (CBD) on the monoamine neurotransmitter in a rat model of methamphetamine ( METH) dependence, and we revealed the potential mechanism underlying the therapeutic effects of CBD. Our research will benefit the treatment of drug dependence in humans. Methods We constructed an animal model based on METH ( 2 mg / kg) exposure and the conditioned place preference procedure. We dissected the nucleus accumbens (NAc), frontal cortex(FC), ventral tegmental area (VTA),caudate putamen(CPu), and hippocampus(Hip). The dopamine, 5-hydroxytryptamine, and norepinephrine contents were separated using high-performance liquid chromatography and quantified with mass spectrometry. Results Repeated METH (2 mg / kg) exposure significantly increased dopamine content and decreased 5-hydroxytryptamine content in the NAc, frontal cortex, VTA, caudate putamen, and hippocampus but increased norepinephrine content in the NAc and VTA of rats. Pretreatment of rats with CBD (10, 20, 40, 80 mg / kg) dose-dependently reduced the impact of METH on these monoamine neurotransmitters. Conclusions CBD may attenuate the reward effect of METH by maintaining the homeostasis of monoamine neurotransmitters in the reward-related brain regions of rats.

    参考文献
    相似文献
    引证文献
引用本文

沈宝玉,任雁明,杨根梦,于 浩,董文娟,洪仕君,张瑞林.大麻二酚减轻甲基苯丙胺诱导的大鼠单胺类神经递质改变[J].中国比较医学杂志,2022,32(11):18~25.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2022-02-28
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2023-01-18
  • 出版日期:
自2024年1期开始,杂志参考文献改为中英文对照,具体格式要求可置下载中心查看!
关闭