Establishment of Polymerase Chain Reaction for Helicobactor sp.and PCR Assay in SD Rats and Field Voles
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Q75 R683.5

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    Abstract:

    Oligonucleotide primers were desi gned from Helicobactor sp. regions of the 16 s rRNA gene that are conserved a mong members of the Helicobactor genus.The assay amplified the expected 374 bp product with P7P8 and 375 bp with H1H2,but no fragment was observed with prim ers P5P6 and P9P10.The specificity of the reaction was determined by testing DNA extracted from Helicobactor of experimentally immunosuppressive rats and Escherichia coli.,Hemolyticus streptococcus,Pasteurella pneumotropica. A produc t of the expected size was generated with Helicobactor, but not with other ba cteria.PCR assay was used to detect Hilicobactor infections in Field Voles,w ith positive 10 samples out of 57 fecal pellets.In conclusion,16S rRNA-based PC R established here is useful for sensitive and specific identification of Heli cobacter in infected animals.

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  • Received:
  • Revised:January 05,2001
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