Objective 23 built and refitted laboratory animal barrier system facilities were surveyed in Shanghai.Methods The National Standards for Laboratory Animal Environment and Facility were implemented.Results The results showed most of the air exchange rate,the air cleanness and the amount of air borne bacterium met the standard requirement,while the control room temperature and the relative humidity were often neglected.Proper design and selection of fittings were essential for controlling the noise and the work-illumination.The pressure gradient and the difference in temperature between day and night were often the key obstacles to meet the requirement of standard laboratory animal facilities. Conclusion The National Standards for Environment and Facility should be followed when laboratory animal barrier system facilities were built or refitted.