Objective To study the rhesus monkey model of the spontaneous diabetes for cloning the gene of diabetes (gene transfer,gene knock-out and gene therapy) and discussing the gene function and expression. Methods The GTT (glucose tolerance test) and glycosuria test in breeding rhesus monkeys were used to choose the rhesus monkey model of the spontaneous diabetes. Results and Conclusion 3 monkeys which were abnormal in glucose tolerance test and positive in glycosuria test were screened from 100 monkeys in middle and elderly age.