Pathological Comparison of Two Type of Demineralization Solutions on the Production of Initial Artificial Enamel Carious Lesion
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R783.1

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    Abstract:

    Objective To determine the effect of two type of demineralization solutions on the production of artificial carious lesion in bovine enamel and human enamel in vitro. Methods A partially saturated acidic buffer solution and an acidifed hydroxyethylcellulose gel system were used as decalcifying media for the preparation of initial artificial carious lesion in bovine enamel and human enamel. After demineralization, these enamel specimens was observed by microscope and scaning electron microscope and compared in histological pathology. Results The results indicated all specimens in a partially saturated acidic buffer solution could morphologically form subsurface demineralization, which were the same as the early smooth enamel caries. Lesions in bovine teeth were pathologically similar to those in human teeth, except that the lesions depth was deeper than those in human teeth. However, In gel, regular subsurface lesions were hardly produced in all specimens. They were either eroded or dissolved. Conclusion It was concluded that caries-like lesions could be consistently produced in bovine enamel with a partially saturated acidic buffer solution. So this demineralization system can be use for reproducible preparation of artificial carious lesions in dental enamel.

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  • Received:
  • Revised:May 21,2007
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