ObjectiveTo establish a human colorectal cancer model on the chick embryo chorioallantoic membrane(CAM) and study its characteristics of angiogenesis.Methods Human colorectal cancer cell line HT-29 at different concentrations were inoculated on the CAMs. To observe the factors affecting the survival of the transplanted human colorectal cancer, growth characteristics of the transplanting tumor and the characteristics of angiogenesis.ResultsThe transplantation tumor model of human colorectal cancer in CAM was successfully established. It was found that transplantation tumor was easy to grow and it showed strong angiogenesis effects.Conclusions It is feasible to establish a transplantation tumor model of human colorectal cancer on CAM. The model can be easily duplicated, which provides a useful animal model for studying the angiogenesis and biological properties of colorectal cancer and can be used in the research of the drug selection for human colorectal cancer.