Objective To measure the organ weights, blood physiological and biochemical parameters of KK/Upj-Ay/J. Methods KK/Upj-Ay/J mice at five and ten weeks of age were selected, and the organ weights, blood physiological and biochemical parameters were observed. Results Parts of the organ weights, blood physiological and biochemical parameters of different ages and sexes were significant differences. The fasted blood glucose of KK/Upj-Ay/J mice reached 7.0mmol/L at 10 weeks of age. Conclusion The results show that the organ weights, blood physiological and biochemical parameters are affected by age and gender of KK/Upj-Ay/J mice. The fasted blood glucose reached the diabetes level at 10 weeks of age.