Objective To investigate the effect of SWF on the behaviors and striatal levels of amino acids neurotransmitters of LID model rats,and to explore the effect of SWF on the mechanism of dyskinesias. Methods SD rats were injected with 6-hydroxydopamine (6-OHDA) to produce hemi-lateral nitro-striatum lesion. 6-OHDA lesioned rats were daily given L-DOPA, or L-DOPA plus SWF at different doses. Sham group was also established. All rats were treated for 22 days, once per day. During the treatment, abnormal involuntary movements (AIMs) were evaluated. On 22nd day, in vivo microdialysis was used to collect striatal extracellular fluid as the rats were in sober and free-moving condition. All dialysates were analyzed by high performance liquid-fluorescence (HPLC-FLD) detection. Results After treatment of L-DOPA, 6-OHDA lesioned rats developed AIM. AIM scores of L-DOPA-treated rats were higher than the sham-operated rats (P < 0.01). L-DOPA plus high dose of SWF-treated rats had lower AIM scores than L-DOPA-treated rats (P < 0.05). On 22nd day, in L-DOPA-treated rats, striatal levels of glutamate and GABA increased (P<0.05) than sham group. 60 min after last treatment, levels of glutamate and GABA in L-DOPA-plus SWF-treated rats were significantly lower than the sham-operated and L-DOPA plus SWF-treated rats (P < 0.05, P < 0.01). Conclusions The findings in this study suggested that as SWF was combined, side effects of L-DOPA mitigated as AIMs scale rating were reduced in LID rats. SWF protected 6-OHDA lesioned rats from LID by regulating abnormal striatal concentrations of glutamate and GABA.