Objective To investigate the synergism and attenuation effects of Pini Pollen on paclitaxel and cisplatin (TP) chemotherapy in model mice. Methods Forty-eight model nude mice with H456 cell line xenograft were divided into 8 groups:model group, TP chemotherapy group, Pini Pollen (150, 300 and 600 mg/kg)group and TP chemotherapy plus Pini Pollen (150, 300 and 600 mg/kg)group. The tumor inhibition rates, body weight, food intake, hematology and blood biochemistry indexes were selected to evaluate the synergism and attenuation effects of intragastric administration of Pini Pollen (150,300,600 mg/kg) on TP chemotherapy. Results Compared with the TP chemotherapy group, the tumor inhibition rates, body weight, food intake, white blood cell count were increased and liver and kidney function damage were alleviated significantly in the TP chemotherapy plus Pini Pollen groups. Conclusions Pini Pollen has a significant synergism and attenuation effects on TP chemotherapy.