The environmental health and safety (EHS) management system is a unified management system that combines the organizational structures of environment, health, and safety, and the related responsibilities, processes, and resources. Biosafety is an important part of the EHS management system. This review summarizes the EHS management system, the occupational risk analysis of laboratory animal practitioners, and biosafety training. Moreover, it describes in detail the importance of biosafety management and training in the EHS management system of laboratory animal practitioners.