Objective To observe the effect of Shenfukang VI decoction combined with prednisone on Adriamycin nephropathy model rats and their glomerular electrostatic barrier, to investigate the mechanism of action of Shenfukang VI in adriamycin nephropathy. Methods Rats were randomly divided into a normal group, a model group, a Chinese medicine group, a prednisone group, and a combination group. Except for the normal group, adriamycin was injected into the tail vein to establish an adriamycin nephropathy model, and the other groups were given the corresponding drugs. The 24?h urinary protein level of each group was determined every week. Four weeks after administration, serum albumin, renal pathological changes, and PCX, HSPG, and HPA protein expression levels were determined. Results Compared with that in the normal group, the 24?h urinary protein level increased in the model group ( P < 0. 05). Compared with that in the model group, the 24?h urinary protein level was decreased in the drug intervention group ( P < 0. 05). In addition, optical microscopy revealed mild hyperplasia in the membrane cells and stroma, the protein tube type was found in the renal tubules, and renal interstitial edema was severe in the model group. The degree of pathological change in renal tissue was significantly reduced in each treatment group. The expression of PCX on GBM was not continuous and the expression of HPA on glomerular endothelium and epithelial cells was high in the model group. Compared with that in the model group, the expression of PCX in renal tissue was increased ( P < 0. 05), the expression of HSPG showed no significant difference ( P > 0. 05), and the expression of HPA was decreased ( P < 0. 05) in the other treatment groups. Conclusions Shenfukang VI decoction can improve the general condition of rats and reduce the 24?h urinary protein level. This decoction can also reduce the severity of kidney disease. This may be related to lowering the expression level of HPA and raising the expression level of PCX protein to improve the glomerular electrostatic barrier.