Experimental animals have made tremendous contributions to medical research. However, the absence of education on the welfare of experimental animals has resulted in poor ethical awareness and a lack of scientific understanding on this issue among medical graduate students. As a result , they may fail to treat experimental animals appropriately and can not scientifically, rationally, and humanely use these animals to carry out scientific research. To enhance the ethics of medical graduate students, medical colleges and universities should standardize the curriculum and strengthen the knowledge and education of experimental animal welfare; explore qualification certificates for employment and normalize the system of research permits; follow the 3Rs’ principle and ensure experimental animal welfare during medical research; strengthen practice supervision; and make ethical reviews a priority. This should improve the behavior regarding the use of experimental animals among medical graduate students.