Objective To investigate the effects of wogobin on the proliferation, migration, and invasion of gastric cancer cells and their molecular mechanisms. Methods Different concentrations (20, 50, and 100 mol/ L) of wogobin were used to treat the hepatoma cell line SGC7901 cells for 24, 48, and 72 h. In the blank control, no treatment was performed. MTT assay was used to determine the cell proliferation. The migration ability of cells was observed by a scratch test. The Transwell method was used to observe cell invasiveness. Cell cycle and apoptosis were determined by flow cytometry. The mRNA and protein expression levels of cellular MMP2, MMP9, ICAM-1, and TIMP2 were determined by RT-qPCR and western blotting, respectively. Results MTT assay showed that the different concentrations of wogobin inhibited the hepatoma cell line SGC7901 in a concentration- and time-dependent manner. After 48 h, wogobin arrested the cell cycle at S-phase and induced the apoptosis in gastric cancer cells ( P <0. 05). Transwell assay showed that wogobin inhibited the invasion of the SGC7901 cells in a concentration-dependent manner. The RT-qPCR and western blotting analyses showed that wogobin inhibited the mRNA and protein expression of MMP9, MMP2, and ICAM-1 in SGC7901cells, but upregulated TIMP2 ( P <0. 05). Conclusions Wogobin inhibites the proliferation, migration, and invasion of the hepatoma cell line SGC7901 cells and induces its apoptosis.