Objective To provide a reference for improving the quality of laboratory animals in Sichuan Province,we reviewed and summarized the results of microorganism and parasite sampling in laboratory animals in the Sichuan area from 2011 to 2015. Methods We sampled and tested the laboratory animal production units in Sichuan Province, and reported on these using current national laboratory animal guidelines. In addition, we analyzed the quality of laboratory animals of different microbiological grades in recent years within Sichuan Province, including mice,rats, guinea pigs,rabbits, dogs, monkeys, and piglets. Results the sample sizes and inspectial frenqency annually of animal samples in these 4 years were basically stable. In 2012, helminths were detected in SPF rats in two units, with a positive rate of 22. 5%. The positive rates of Staphylococcus aureus were 5. 0%, 5. 0%, 40. 0%, and 11. 4% in different units for 4 consecutive years. In 2015, parvovirus RV and H-1 in rats were positive in three units, with positive rates of 34. 3% and 28. 6%, respectively. In 2012 and 2013, ectoparasites were detected in different units of SPF mice, with positive rates of 7. 1% and 12. 9%, respectively. In the helminthes inspection,the positive rate of the samples was 10% in 2012,1. 4% in 2013 and 7. 5%in 2015.In 2011, Klebsiella pneumonia was positive in one unit, with a positive rate of 1. 4%. The guinea pigs samples fet the standards totally in recent years. In 2013, Toxoplasma gondii was positive in rabbits in one unit, with a positive rate of 10%. In 2012, the positive rates of pathogenic dermal fungi and Toxoplasma gondii in dogs from two units were 5. 0% and 2. 5%, respectively. In 2011, the monkeys in two units tested positive for pathogenic dermal fungi, with a rate of 9. 5%. In 2011 and 2012, the same single unit tested positive for Shigella spp. for two consecutive years, with rates of 2. 4% and 2. 5%, respectively. The cercopithecine herpesvirus type 1 was found in one unit in 2012, with a positive rate of 2. 5%. In 2015, in the first examination of piglets, only Brucella and Toxoplasma were tested, with a negative result.Conclusions Quality monitoring and sampling inspection of laboratory animals are important to guarantee the quality of laboratory animals. They also play a vital role in improving the quality of laboratory animals in Sichuan Province, reducing the differences between Sichuan and developed area, and promoting the development of the Sichuan laboratory animal industry.