Effects of risedronate sodium combined with simvastatin on tail suspension-induced bone loss in mice
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(1. KaiLuan General Hospital, Tangshan 063000, China. 2. North China University of Science and Technology, Tangshan 063000. 3. KaiLuan Linxi Hospital, Tangshan 063000)

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R-33

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    Abstract:

    Objective To compare the efficiency of single or combined application of simvastatin and risedronate sodium on a disused osteopenia rat model with tail suspension. Methods Thirty 12-week-old C57BL6 mice were assigned to five groups of six mice each: control (A), tail suspension (B), simvastatin treatment (C), risedronate sodium treatment (D), and combined treatment (E). All but group A received tail suspension, and the groups were treated with the vehicle, simvastatin (5 mg/ (kg·d)), risedronate sodium (1 mg/ (kg·d)) or a combination of both, respectively. All mice were sacrificed after 3 weeks. Micro-CT was performed on the left tibia to determine the bone mass and microstructure of both the trabecular and cortical bones. Three-point bending test was used for biomechanical analysis of the left femur. The right tibias were subjected to RNA extraction and PCR analysis of OPG and RANKL expression, and the right femurs were used for protein level detection by western blot. Results Micro-CT: BV/ TV and Tb.N in group B were significantly lower than those of the other groups ( P < 0. 05), the Tb.Sp in group B was significantly higher than that of the other groups. BV/ TV in groups C and D were significantly lower than those in groups A and E, while Tb. Sp in groups C and D was significantly higher than that in group A ( P < 0. 05). ② Biomechanical test: maximal loading and the elastic modulus in group B were significantly lower than those of groups A and E ( P < 0. 05). ③ PCR: the mRNA level of OPG in groups C and E was significantly higher than that in group B ( P < 0. 05), and RANKL was expressed significantly higher in group B than that in group A ( P < 0. 05). ④ Western blot: the protein level of OPG in group A was markedly higher than that in the other groups ( P < 0. 05), OPG in group E was significantly higher than that in group B ( P < 0. 05), and the protein level of RANKL in group A was significantly lower than that in the other groups ( P < 0. 05). Conclusions Tail suspension caused bone loss and deterioration of bone quality in mice, and simvastatin combined with risedronate sodium treatment showed more substantial attenuation of these effects than treatment with either drug alone.

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  • Online: December 10,2019
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