Effects of thrombopoietin on adriamycin-induced cardiomyocyte apoptosis in rats with heart failure based on the TGF-β1 / Smad3 signaling pathway
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1. Department of cardiovascular medicine, general hospital of shenzhen university, Shenzhen 518005, China. 2. Department of internal medicine, Baoan Women’s and Children’s Hospital, Shenzhen 518000. 3. Department of cardiovascular medicine, the first hospital of lanzhou university, Lanzhou 730000

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R-33

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    Abstract:

    Objective To investigate the effects of thrombopoietin on adriamycin-induced cardiomyocyte apoptosis in rats with heart failure based on the TGF-β1 / Smad3 signaling pathway. Methods Rats were randomly divided into control, model, treatment, and inhibitor groups. Rats in the model, treatment, and inhibitor groups were injected with doxorubicin to establish a rat heart failure model. Rats in the treatment group were intraperitoneally injected with 5 μg / kg thyroperoxidase (TPO) daily and rats in the inhibitor group were intraperitoneally injected with 1. 2 mg daily / kg SB431542. TUNEL staining, immunofluorescence, and western blot were used to detect the cell apoptosis of cardiomyocytes, and the expressions of caspase-3, TGF-β1, and p-Smad3. Results Compared with the control group, the cell apoptosis rate of myocardial tissue cells in the model group was significantly increased and the expressions of TGF-β1, p-Smad3 and caspase-3 were significantly increased. Compared with the model group, the apoptosis rate of myocardial tissue in the treatment and inhibitor groups and the expressions of TGF-β1, p-Smad3, and caspase-3, were significantly decreased. The differences were statistically significant compared with the model group ( P < 0. 05). Conclusions TPO reduced the apoptosis rate of cardiomyocytes induced by doxorubicin in rats. The mechanism may be related to the inhibition of the TGF- β1 / Smad3 signaling pathway

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History
  • Received:September 22,2019
  • Revised:
  • Adopted:
  • Online: August 19,2020
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