Objective Animal models of low immunity were established in mice using a simple and rapid method. Methods Cyclophosphamide was administered orally at a dose of 100 mg / Kg, hydrocortisone was administered orally at a dose of 80 mg / Kg, a 60% ethanol solution was administered orally at a dose of 20 ml / kg, and skin was irradiated by UVA (long wave) at a dose of 10 J/ cm2 . Each group was treated with medicine for 3 consecutive days. The body weight of mice was measured before and after the test, and the levels of IgG, IgA, and IgM in the serum of mice was determined. In mice, the proportions of CD19+ B cells in peripheral blood lymphocytes and CD3+ T cells in peripheral blood lymphocytes were measured by flow cytometry. The peripheral blood cells of mice were analyzed by an automatic hematology analyzer. Results Immunosuppressive mouse models using cyclophosphamide or hydrocortisone were established. Immunosuppressive models using 60% ethanol or ultraviolet irradiation were not established, and require further study. The body weight of mice, the levels of IgG, IgA, and IgM in the serum, B and T lymphocytes in peripheral blood, and the routine analysis of blood in mice can be used as reference indexes for the establishment of models of low immunity in mice. Conclusions A model of immunosuppression established in mice by the oral administration of cyclophosphamide at 100 mg / kg for 3 days was established. Regarding the various detection indexes, body weight, serum immunoglobulin content, and routine blood analysis are simple to perform, rapid, and of low cost with a high detection rate. These can be used as indexes for the routine detection of established animal models