Objective To calculate and analyze blood pressure data and histamine sensitivity of domestic cats on examination of depressor substance, and to provide a reference for the use of cats in experiments. Methods Blood pressure and histamine sensitivity were measured in domestic cats using the test method described in the Appendix of China Pharmacopoeia ( edition 2015) after analyzing the combined anesthesia data. Results Blood pressure and histamine sensitivity measurements were not correlated with body weight, whereas histamine sensitivity was correlated with blood pressure in this study. Furthermore, cats with blood pressure within the range of 16 ~ 18 kPa had a greater capacity for repeat examinations of depressor substances. Blood pressure and histamine sensitivity measurements did not decrease during the first six rounds of experiments. While blood pressure decreased significantly on the seventh and eighth rounds (P < 0. 05), and histamine sensitivity decreased on the eighth round (P < 0. 05), compared with data from the first round, the result still met the requirements of China Pharmacopoeia. Conclusions Blood pressure and histamine sensitivity measurements in cats were relatively stable, suggesting that examinations of depressor substances can be performed repeatedly in domestic cats.