Isolation, culture, and characterization of mouse lung-resident mesenchymal stem cells
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Institute of Laboratory Key Laboratory of Human Disease Sciences, Chinese Academy of Medical Sciences(CAMS); Comparative Medicine Center, Peking Union Medical College(PUMC), Key Laboratory of Human Disease Comparative Medicine, National Health Commission of the People’s Republic of China; Key Laboratory of Human Disease Key Laboratory of Human Disease Models, State Administration of Traditional Chineses Medicine, Beijing 100021, China

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R-33

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    Abstract:

    Objective To establish a stable method for the isolation, culture, and characterization of mouse lung- resident mesenchymal stem cells, which will then be used to study lung repair and regeneration mechanisms. Methods Mouse lungs were obtained via germ-free procedures and were mechanically minced and / or digested with collagenase. Cell morphology was observed by phase-contrast microscopy, cell surface markers were detected by flow cytometry, and cells were induced to differentiate into adipocytes or osteoblasts. Results We successfully isolated and cultured mouse lung- resident mesenchymal stem cells. FACS analysis demonstrated high expressions of Sca-1, CD90, CD29, and CD44, and low expressions of CD31 and CD45. The lung-resident stem cells differentiated into adipocytes or osteoblasts. Conclusions We isolated cells that expressed mesenchymal stem cell markers and had multi-directional differentiation ability.

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History
  • Received:November 05,2020
  • Revised:
  • Adopted:
  • Online: September 26,2021
  • Published: