Objective To observe the effects of Qingre Runzao oral liquid on the numbers of type Ⅱ intrinsic lymphocytes (ILC2s) and their upstream and downstream cytokines in asthmatic mice sensitized by ovalbumin. Methods Female BALB/ c mice were divided into the blank control group, model control group, positive control group (dexamethasone group, 1 mg / (kg·d)) and low- and high-dose Qingre Runzao oral liquid groups (4. 32 and 8. 64 g / (kg ·d)). The asthma mouse model was induced using ovalbumin as a sensitizer, and pathological changes in lung tissue were observed by hematoxylin and eosin staining. Levels of the upstream and downstream cytokines of ILC2s Eotaxin, interleukin (IL)-25, IL-33, thymic stromal lymphopoietin ( TSLP), IL-4, IL-5 and IL-6 were detected in mouse bronchoalveolar lavage fluid ( BALF) using enzyme-linked immunosorbent assay. The expression of IL-33 and IL-5 in lung tissue was detected by immunohistochemical method . Flow cytometry and immunofluorescence staining were used to assess the number and production of ILC2s in lung tissue. Results Compared with the blank control group, the model control group showed pathological changes of inflammatory cell infiltration in the lung tissue and bronchial wall thickening. Furthermore, levels of the upstream and downstream cytokines Eotaxin, IL-25, IL-33, TSLP, IL-4, IL-5 and IL-6 were increased in BALF (P< 0. 01). The expression of IL-33 and IL-5 was increased in lung tissue (P<0. 01), and the number and production of ILC2s was also increased (P<0. 01). Compared with the model control group, both the low and high doses of Qingre Runzao oral liquid significantly improved the pathological injury to lung tissue in asthmatic mice, decreased the expression of upstream and downstream cytokines of ILC2s in BALF (P<0. 01), downregulated the expression of IL-33 and IL-5 in lung tissue (P<0. 05, P<0. 01), and decreased the number and production of ILC2s (P<0. 01). Conclusions Qingre Runzao oral liquid can reduce the numbers of ILC2s and their upstream and downstream cytokines in ovalbumin-induced asthmatic mice. It can also downregulate the expression of IL-33 and IL-5 in lung tissue, thus playing a role in the prevention and treatment of asthma.