Objective To investigate the feasibility and effect of rat epidural anesthesia models by modifying fixation of the epidural catheter. Methods 24 male Wistar rats, used to build epidural anesthesia models, were randomly divided into experimental group (n = 12) and control group (n = 12). After inserted the catheter, the catheter were fixed by some blocks designed with sterile adhesive tape and the catheter connector were replaced by sterile medical tape in the experimental group, while catheter was fixed with wire wound and catheter connector was sutured on the back of the neck in the control group. 0.1% ropivacaine 30ul was injected into epidural space once a day and continued 28 days. Then incidence of postoperative infection, the time to failure of the epidural anesthesia models was recorded and the epidural catheter depth measured on the 28th day. Results No significant difference was noted in the incidence of postoperative infection between two groups (P>0.05). The effective duration of models in the experimental group is significantly longer than that in the control group(26.2±1.7d & 18.5±3.0d，P<0.05). The epidural catheter depth were 1.81±0.07cm in the experimental group, respectively, whereas in the control group, 1.44±0.55cm of the depth were detected, the degree of dispersion is lower than that in the control group(P<0.05). Conclusion Modified catheter fixation can significantly improve the stability and success rate of epidural anesthesia animal models.