Objective To construct pEGFP-C1-BAFF, pEGFP-N1-BAFF, and pIRES2-EGFP-BAFF recombinant plasmids, and to generate establish a SLE disease model of BAFF overexpression zebrafish by microinjection and fluorescence screening. Methods We cloned BAFF full length cDNA encoded by 807bp nuclear acid using spleens RNA of zebrafish by RT-PCR and constructed 3 types of BAFF overexpression plasmids: pEGFP-C1-BAFF,Objective We construct pEGFP-C1-baff, pEGFP-N1-baff, and pIRES2-EGFP-baff recombinant plasmids, and establish a SLE disease model of Baff overexpressed zebrafish by microinjection and fluorescence screening. The aim is to evaluate the meaning of Baff overexpression zebrafish in study of SLE mechanism and drug selection. Methods We cloned baff full length cDNA encoded by 807bp nuclear acid using spleens RNA of zebrafish by RT-PCR and constructed 3 types of baff overexpression plasmids: pEGFP-C1-baff, pEGFP-N1-baff and pIRES2-EGFP-baff. Baff protein expression was estimated by in-vitro cell transfection and Western blotting. Results 3 types of baff overexpression plasmids were examined by agarose gel electrophoresis. Western blotting and in-vitro transfection confirmed the expression of Baff-GFP fusion protein. We also obtained the baff over expression zebrafish by microinjection and fluorescence screening. Conclusion All the plasmid we constructed in our research have been estimated and could be used to generate the disease model of baff overexpression zebrafish which can be manipulated for immune-disease research. pEGFP-N1-BAFF and pIRES2-EGFP-BAFF. BAFF protein expression was estimated by in-vitro cell transfection and Western blotting. Results 3 types of BAFF over expression plasmids were examined by agarose gel electrophoresis. Western blotting and in-vitro transfection confirmed the expression of BAFF-GFP fusion protein. We also obtained the BAFF over expression zebrafish by microinjection and fluorescence screening. Conclusion All the plasmid we constructed in our research have been estimated and could be used to generate the disease model of BAFF overexpression zebrafish which can be manipulated for immune-disease research.