[Abstract] Objective To establish the disease syndrome combined animal model, the WHBE rabbit myocardial ischemia model of phlegm-blood stasis syndrome type, by high fat diet feeding and coronary artery ligation. Methods WHBE rabbits were randomly divided into the normal control (NC) group, myocardial ischemia model (MI) group and myocardial ischemia model of phlegm-blood stastis syndrome (PBSS-MI) group, 6 in each group. The myocardial ischemia of MI group and PBSS-MI group were induced by ligated the coronary artery, and then NC group and MI group were fed with common forage, while PBSS-MI group was fed with high fat forage for 4 weeks. The model establishment and its pathological process of phlegm-blood stasis syndrome were evaluated by examinations on body weight, myocardial enzymes indexes, blood liquid levels, hemorheology and myocardial infarction size (MIS), and correlation analysis by Pearson method. Results Compared with NC, the weight of MI group was slightly lowered, and the serum CK, LDH, TG, plasma viscosity, casson viscosity and myocardial infarction size were significantly increased (P<0.05, P<0.01).While the weight of PBSS-MI group had no obviously lowered, but serum CK, LDH, TC,TG, LDL-C, HDL-C, whole blood viscosity, plasma viscosity, erythrocyte electrophoresis time, casson viscosity and myocardial infarction size were significantly increased (P<0.05, P<0.01). Compared with MI group, TC, LDL-C, HDL-C, high shear rate of whole blood viscosity, erythrocyte electrophoresis time and myocardial infarction size were higher than those of MI group (P<0.05, P<0.01). Correlation analysis showed that MIS and TC, TG and HDL-C in MI group were closely related (P<0.01), and MIS and TC,TG, HDL-C, LDL-C, LDH, CK, high shear rate of whole blood viscosity, low shear rate of whole blood viscosity, erythrocyte electrophoresis time and cassion viscosity in PBSS-MI group were also closely related (P<0.05, P<0.01). Conclusion Disease syndrome combined model for myocardial ischemia model of phlegm-blood stasis syndrome type in WHBE rabbit could be established by high fat diet feeding with coronary artery ligation, and it has related changes between MIS and blood lipids level, hemorheology, which may reflect related biological basis of the traditional Chinese medicine of phlegm and stasis cementation.