选用1～3岁龄的国产恒河猴(Macaca mulatta)22只(其中有2只作为对照)进行了戊型肝炎病毒(Hepatitis E Virus简称HEV)的实验感染.第一代感染两批动物:第一批感染4只,有3只发病,第二批感染4只全部发病.而后用第二批感染猴的粪便和肝脏悬液作为第一代,又连续进行传代实验。第二代传代选用的4只猴也全部发病.用肝悬液传代的1只猴也发病.第三代传代选用7只猴,有5只猴发病其中有1只猴(M-34号)是用第二代传代猴的胆汁感染的.所有发病猴血清丙氨酸转氨酶(ALT)水平均有不同程度的升高,一般高于正常值的3～6倍,最高可达10倍左右。肝活检可见肝细胞炎症和坏死。猴的粪便标本用免疫电镜(IEM)检测可见到直径为27～32nm的病毒颗粒。
An experimental model of hepatitis E was established in rhesus monkeys (Macaca mulatta). First-passage animals were inoculated with two different stool suspensions obtained from human patients with hepatitis E which originated from Xinjiang and Senyan, respectively. Three of four monkeys were infected,27~34 nm diameter virus-like particles were found in feces of infected animals by immune electron microscopy(IEM). Liver biopsy of the three monkeys showed histopathologic changes of acute-phase hepatitis E. Second-passage animals were inoculated with HEV in both stool and liver suspension from a first-passage monkey ( M-16), including one animal inoculated with liver suspension. Four of five animals were infected. The third-passage animal was infected with the secondpassage monkey both stool suspension and bile. Five of seven animals were infected.