实验感染恒河猴建立庚型肝炎动物模型的研究
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Q813 R512


Study on the Experimental Model of Hepatitis G of Macaques
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    摘要:

    用庚型肝炎病毒(HGV)人工感染恒河猴建立庚型肝炎动物模型在我国尚属首例。该实验用了5只恒河猴(2其感染.1只传代.2只对照)。本实验采用:逆转录PCR法、间接ELISA法、酶速率法、肝组织光镜动态病理方法作为检测手段。其结果是:研究的2只猴都出现HGV-RNA、HGV-IgG阳性,持续时间50周以上。感染第10周肝组织出现急、慢性病毒性肝炎病变.并与人庚型肝炎病变基本相同,而血清转氨酶(ALT)变化不明显。用感染后第7周猴子的阳性血清传代感染另一只猴:用同样的检测方法、内容,其结果与供血清猴的变化特征相同。实践证明.实验感染恒河猴建立庚型肝炎动物模型的研究首次获得成功。

    Abstract:

    Hepatitis G virus was successfully transmitted to macaqucs.Five animals were inoculatcd with scrum from a paticnt whose serum was HGV RNA positive by RT PCR.The serum and bioptic liver tissues were examined by RT PCR,ELISA and light microscope respectively.The results showed that 2 macaques with normal ALT levels had seroconversion of HGV RNA and anti HGV IgG for over 50 weeks and their liver tissues had the changes of acute and chronic hepatitis which were very similar to those of patients with hepatitis G.The HGV positive serum was subpassaged in another macaque at the 7th week after transmission.The changes was as same as that of the first passage animals.

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张文志 张贞姬 刘学领 荣俊清 貌盼勇 赵景民 毛远丽.实验感染恒河猴建立庚型肝炎动物模型的研究[J].中国比较医学杂志,1998,(3):152~156.

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