Q78 R730
国家自然基金重点项目!(396 30 10 0 )资助课题
通过提取小鼠细胞总RNA，采用RT-PCR的方法，扩增了连接蛋白Cx43的cDNA整个氨基酸编码区域，并将其克隆到了pGEM质粒中的EcoR．I位点。对序列的分析显示其有1146bp，编码382个氨基酸。与已有大鼠、牛及人相应序列比较，存在部分差异。该重组质粒工作的完成。为其表达、调控等方面的研究奠定了基础。
Connexin genes are suggested to be a group of potential trmor- suppressors. We obtained a 1146bp cDNA fragment of NIH mouse connexin43 by areve rse transcription-DNA polymerase chain reaction. This cDNA fragment was then clo ned into pGEM plasmid.Compared with the rat, bovine and human connexin43 genes, there were some varieties among them.
胡根林,吴瑞英,姜槐,郭汉身.小鼠细胞间隙连接蛋白基因的克隆和序列分析[J].中国比较医学杂志,2000,(1):1~4.
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