In this paper,the effect of mutagenicity of the Jiuxin Fumai Injection was investigated.The results showed that Jiuxin Fumai Injection did not induce positive mutation of strains TA 97 , TA 98 ,TA 100 and TA 102 in the Ames test.The rates of CHO chromosome aberration of Jiuxin Fumai Injection were negative range(<5%).The results of micronucleus assay of polychromatic erythrocytes in mice of Jiuxin Fumai Injection at 3 doses of 2.0 g/kg, 4.0 g/kg, 8.0 g/kg would not be to induce increase of its micronuclei.It was proved that Jiuxin Fumai Injection had no any mutagenicity in this experiment.