As enlargement of poultry industries, demand of SPF eggs has been increased quickly. 2 000 breeder eggs(l800 of female line and 200 of male line) were introduced from America in order to meet marketing requirements in 1995. New colony had been hatched and maintained in separated barrier system until completing animal health tests twice at 10 and 16 weeks old of chickens. After several generations, the production performances of new colony were much better than original data, particular in laying rate and fertility, from 83% and 93% to 85% and 98% separately.