Objective The aim of this study is to examine the genetic characteristics of the IRM-2 mouse which is a new inbred strain mouse hybridized by the ICR(paternal strain) with 615(maternal strain) and has progressed to 38th generation since 1990. Methods Genetic monitoring of the IRM-2 inbred strain mouse was performed by skin grafting test, coat color test and biochemical marker analysis. Results The skin grafting test showed that no dropping graft was found during 100 days after transplanting, implying the IRM-2 was of isohistogenicity. Analysis of coat color genes indicated that hair color of first generation hybrid crossed between F38 and BALB/c was cassia bark as same as the IRM-2, suggesting that the coat color genes of the IRM-2 of were homogenous. The gene type is AAbbCCDD. Measuring of 25 and 13 biochenmical markers gene loci on 13 chromosomes was performed by electrophoresis for F23 and F38 respectively. The results revealed that all observed gene loci were homogenous without any polymorphism and mutation. Conclusion The IRM-2 inbred strain mouse is high homogenous in the genetic characterishcs.