Q95-337 S858
广州军区广州总医院科研基金资助
实验选用怀孕中国1号小型猪6头，采用隔离器剖腹产微生物净化方法，生产仔猪51头，因体重过轻、死胎等淘汰7头，成活44头，手术成活率86．3％；30d断乳成活36头，离乳率81．8％。在隔离器内饲喂10周，术后21d、42d、63d分别用硫乙醇酸盐培养基(USP)，营养肉汤(NB)，萨布罗氏肉汤(sB)检测粪便、饲料、饮水、进排风口、除粪口部厌氧菌、需氧菌、霉菌，结果皆为阴性。对剖腹产前母猪、45d仔猪进行猪巴氏杆菌、丹毒、口蹄疫、猪瘟、猪细小病毒、弓形体6种致病病原体检测，结果阴性，仔猪达到SPF标准。
In this experiment we selected six Chinese No.l miniature pigs to produce 51 pigs by laparohysterectomy-birth in the isolators, 7 pigs were shifted out because of too light body weight or dead fetus. 44 pigs existed with the surgery etistense percentage of 86.3%. After stopping lactation 36 pigs existed (81 .8%). In 10 weeks of feeding period, we checked and tested feces, feeding material, drinking-water, wind inlet /outlet and feces outlet for the anaerobic bacteria, aerobic bacteria, fungus, separately at 21d, 42d, 63d by the methods of USP, NB, SB, and all the test results were negative. Pig Pasteurella bacteria, Swine erysipelas bacteria, foot and mouth disease (FMD) virus, swine fever virus, pig mini-virus, toxoplasma were detected with all the negative results both on the mother pig before laparohysterectomy-birth and on the new-born pigs at 45d after the birth. The new-born pigs fulfill the SPF standards.
詹纯列,徐本法,李建军,李权超.小型猪剖腹产人工哺乳净化[J].中国比较医学杂志,2000,(4):213~216.
复制