Objective In order to master the diagnosis method of vitamin D intoxication by studying vitamin D of content diets of laboratory rats and mice.Methods BALB/c mice were fed with different vitamin D content diets as experimental group and control group, pathological observations were made and blood phosphorus and blood calcium were determined. Results The rate of myocardial calcareous deposit is 53.5% in the experimental group and 0 in the control group. Blood calcium value of the former is 1.6 times that of the latter; blood phosphorus value shows no significant difference between two groups.Conclusion The decreasing of weaning rate, the myocardial calcinosis and the rising of blood calcium value in laboratory rats and mice are caused by superfluous vitamin D intake.