目的 观察不同正加速度( Gz)作用后大鼠血清中尿素氮(BUN)、肌酐(Cr)、碱性磷酸酶(AKP)和Ca2 浓度及肾脏病理改变。方法 36只雄性wistar大鼠随机分为6组，每组6只。A组为 1Gz，B组为 5Gz，C组和D组为 7Gz，E组和F组为 9Gz。B组、C组和E组在第3次加速度作用(每次3min，间隔40min，共3次)后6h处死。D组和F组在24h后处死，测上述指标。结果 以A组为对照组，对照组的BUN、Ca^2 和AKP分别为(9．2土0．71)mmol/L、(0．57土0．29)mmol／L和(460土74．06)U／L，C组和E组的BUN[(10．18土0．58)mmol／L、(11土0．35)mmol/L]有显升高(P＜0．05，P＜0．01)．B组、C组和E组的Ga2 [(2．5土0．54)mmol／L、(3．1土0．28)mmol／L和(3．07l0．12)mmol／L]显降低(P＜0．01、P＜0．05和P＜0．01)。E组的AKP(543．17l2．27)U／L有显升高(P＜0．05)。BUN是恢复至正常最快的肾功能指标。受 7Gz和9Gz重复作用后，C组和E组电镜下见肾小管上皮细胞，线粒体有病理变化。结论 正加速度对大鼠肾功能和超微结构有损害作用。
Objective To observe effect of different +Gz on renal function and its ultrastruture in rats.Methods Thirty-six male Wistar rats were randomly divided into six groups ( n =6 for each group).Group A served as ground control.Group B was exposed to +5 Gz while group C and group D exposed to +7 Gz, group E and group F exposed to +9Gz for 3 min in animal centrifuge (3 times,with 40 min interval in between).Blood samples of group B,C and group E were collected 6 h after +Gz exposure,and those of group D and group F collected 24 h after exposure,for the measurement of blood urea nitrogen (BUN), creatinine (Cr),alkaline phosphatase(AKP) and Ca 2+ levels. At the same time,the left kidney was taken for microscopic and electron microscopic examination.Results In comparision with group A, BUN in group C and group E were significantly elevated ( P <0.05). Ca 2+ levels in group B,C and group E were significantly decreased ( P <0.01, P <0.05 and P <0.01).AKP in group E was also significantly increased ( P <0.05).In electron microscopic examination, the pathological changes of mitochondria of glomeruli and renal tubules were found in rats after repeated +7 Gz and +9 Gz exposure.Conclusion Repeated +7 Gz and +9 Gz exposure may exert harmful effect on renal function and ultrastructure in rats.
王喆,戴雨,笪冀平,戴大江.正加速度对大鼠肾功能和超微结构的影响[J].中国比较医学杂志,2003,(1):10~12.
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