Objective To determine the hEPO transgene on the chromosomes of three transgenic goats obtained by zygote microinjection.Method\ Fluorescence \%in situ\% hybridization technique was used to detect the hEPO transgene.The biotin labeled probe was hybridized with the chromosomes of transgenic goat,normal control goat and human's.Results\ By signal amplification and DAPI staining,one pair of signals could be observed obviously on different chromosomes of these transgenic goats,while no signals produced on normal goat and human chromosomes.Conclusion\ hEPO transgenes were integrated into the chromosomes of these three goats with single site,and the integration was random on the goat chromosomes.