Objective To evaluate the effect of seawater immersion after open abdominal trauma on several plasma enzymes. Methods Sixty Wistar rats were divided into two groups. A model of an open abdominal injury was established. Control group(n=30) was simple abdominal trauma. Experimental group(n=30) had seawater immersion after chest trauma. Blood samples were taken at three different time intervals for assessing creatine kinase (CK), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Results The plasma CK, LDH, ALT and AST were significantly higher in experimental group than that in control group. Conclusion Plasma enzymes including plasma CK, LDH, ALT and AST have significantly changes in experimental animals and more severe with time.