Objective To explore and improve operative management of pancreas transplantation in miniature swine. Methods The 40 miniature swine were divided into donors and recipients and pancreas transplantation was performed 20 times in all. Miniature swine were anesthetized with ketamine and maintained with ketamine and etomidate. Meanwhile, mean artery pressure(MAP), central venous pressure (CVP) and blood gas (BG) were monitored. And the 400-800 ml blood was obtained from abdominal aorta of donor and transfused to recipient in the operation. Dexamethasone and dopamine were given in the reopening of inosculated blood vessels. Results The rate of successful operation was 90%. Recipient's MAP were significantly decreased after the reopening of inosculated blood vessels,compared with MAP before the reopening(P<0.05). Other monitored data had not markedly changed. It was more superior and convenient to obtain blood from abdominal aorta than inferior vena cava of the donor. The average transfusion was 400 ml to a recipient and injected dexamethasone and dopamine had contributed to stabilization of circular system in the reopening of inosculated blood vessels. Conclusion Enhancement of monitoring and management of circular system, timely transfusion and stabilization of blood pressure could improve the rate of successful operation and were crucial for pancreas transplantation in miniature swine, especially after the reopening of inosculated blood vessels.