The macaque was anaesthetized by injecting ketamine hydrochloride in muscle before fixing the head of the monkey in the stereotactic frame. And then, The craniotomy for the monkey could be done. The result showed no complications and deaths due to craniotomy in all these monkeys with operation. The method is a stable anaesthetization to make a model of head injury or to transplant the stem cells under exposure of the parietal lobe, occipital lobe, temporal lobe or the posterior of the frontal lobe.