Twenty microsatellite DNA markers were selected from poultry genome in this study. The genomes of 100 Lingnan yellow chickens were analysed. the results from this study indicated that 13 of 20 microsatellite DNA markers were polymorphic.The number of alleles was 4-7, the average value of polymorphism information content was 0^6109 and the average value of heterozygosity was 0^6712.;