Lewis大鼠子宫内膜异位症模型复制
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R711.71 R-332

基金项目:

湖南省卫生厅基金资助 ( 0 0 0 5 4)


A Lewis Rat Model of Endometriosis
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    摘要:

    目的 建立子宫内膜异位症 (EMT)的Lewis大鼠动物模型。方法 取Lewis大鼠 4 0只 ,分 3组 ,Ⅰ组 :用手术移植的方法把自体子宫组织块移植到腹膜、肠系膜上 ;Ⅱ组 :分别把子宫角近侧端和远侧端的子宫组织块移植于腹膜上 ;Ⅲ组 :移植同样大小的脂肪组织于腹膜 ,于术后 4、8周 ,再次开腹 ,观察存活情况和病理学变化。结果 腹膜和肠系膜病灶的面积和成活率差异无显著性 (P >0 0 5 ) ,移植远侧端的异位病灶的面积和成活率大于移植近侧端的异位病灶的面积和成活率 (P <0 0 5 ) ,移植物外观呈囊状 ,表面有血管 ,镜下见子宫内膜上皮细胞、间质细胞和腺体 ,类似于正常的内膜 ,但间质细胞层较薄 ,腺体减少。结论 该模型生长快 ,成功率高 ,为研究子宫内膜异位症的较理想的动物模型

    Abstract:

    Objective To set up a rat animal model of endometriosis. Methods Lewis rats were divided into three groups: 1. The uterine tissues of Lewis rats were autotransplanted to the surface of the peritoneal cavity and the mesenteries.2. The distal or the proximal uterine tissue squares of rat uterine horn were sutured to the peritoneal cavity. 3. The same fat squares were transplanted into the peritoneal cavity,then pathological changes were observed after four or eight weeks. Results No difference was noted in the area and survival rate between implant to the peritoneal cavity and that to the mesenteries(P>0.05). Uterine distal squares that were autotransplanted to the surface of the peritoneal cavity were larger and more easy to success(P<0.05). The endometrial implants in the rats became cystic structures that were composed of endometrial gland and stroma.The macroscopic and histologic appearance was consistant with endometriosis. Conclusion The autotransplanted surgically model of endometriosis would grow stably with easy success. It could be a suitable animal model.

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吴献青 林秋华 丁晖 童敏. Lewis大鼠子宫内膜异位症模型复制[J].中国比较医学杂志,2004,(1):27~30.

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  • 最后修改日期:2002-08-05
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