Objective To compare the permeation condition of ligustrazine hydrochloride for skin of SPF laboratory animals BALB/c nude mice,KM mice and SD rats in order to give evidence of fit skin of SPF animals in the osmosis experiment in vitro. Methods An experiment installation of extrinsic permeation skin of the two room diffuse pool was used for the skin to detect the osmosis coefficient and the effect of store depot of ligustrazane hydrochloride in defferent kinds of animals. Results There was a remar kable difference in the absorbent effect of permeation skin for ligustrazine hydrochloride between back and abdomen in the three kinds of animals, among them osmosis coefficient was the highest in back and abdomen of the nude mice. The back skin absorbent effect of permeation skin was better than the abdomen skin in same kind of animals. Deposit function of BALB/c nude mice skin was better than the skin of SD rats. Deposit function of back skin was better than the abdomen in every animal. Conclusion The back skin of nude mice is more suitable for the experiment of extrinsic permeation in usual SPF laboratory animals.