Objective To acknowledge the Beagle's normal levels of hematology and blood biochemicals. Methods 10 blood biochemicals and 9 blood routine values of Beagles from different origins and ages were examined by BSALC partial blood automated chemical analyzer and German made Medicon 4000 fully automated hematology analyzer. Results The results were coordinated with those reported in the published articles, fluctuating in the rang of normal levels except the high level of ALP, compared with the ordinary human specimen. Conclusion The experimental animal of different origins and ages has a relation with the results. It's necessary to pay close attention to the coordination of the origins and ages of the experimental animals during the experiments.