Objective To compare the reactivity of imported and domestic monoclonal antibodies against H-2 antigens of inbred strain mouse. Methods The percentage of quantity of obituary cells (positive cells) was acquired by using microcytotoxicity test. Result The imported antibodies H-2D~b(27-11-13), H-2D~d(34-5-8S), H-2D~k(15-5-5.3), H-2K~k(16-3.22.4) reacted to the homologous antigens and the death rate of the spleen cells was more than 60%. The domestic antibodies H-2D~b(2), H-2D~d(4), H-2D~k(32), H-2K~b(33), H-2K~d(31), H-2K~k(23) induced death rate of spleen cells was also over 60%. The reaction of both imported and domestic monoclonal antibodies against H-2 antigens of inbred strain mouse was in consistency. Conclusion The domestic monoclonal antibodies against H-2 antigens of inbred strain mouse can also be applied to monitor the mouse's H-2D and H-2K in mice.