Objective To investigate the cardioprotective effects of delayed preconditioning of allitridium on experimental acute myocardial infarction in rabbits. Methods Rabbits were pretreated using allitridum (1 mg/Kg or 0.5 mg/Kg). The percentage of the weight at infarction and at risk (I/R), the myocardial morphological changes were measured 24 h later to determine the protective effects against experimental ischemia/reperfussion injury. Results It was observed in hearts treated with 1 mg/Kg allitridium that I/R area decreased 30.62% and the ultrastructure was obviously improved than the compared group. Conclusion Allitriuium can mimic the delayed cardioprotection on experimental acute myocardial infarction in rabbits. Allitridium has potential value in treating coronary heart diseases.