Objective To establish the methods for the detection of antibody to mouse Toxoplasmosis. Method Antigen and positive serum of Toxoplasmosis were prepared, and the conditions of IFA and ELISA determined. Two pairs of primers were designed and synthesized based on the sequence of Toxoplasma gondii B1,and the PCR was constructed. Result The positive rates detected by IFA and ELISA were significantly higher than that by indirect haemagglutination (IHA) and antibody titres in ELISA were higher than in IFA. Conclus The IFA and ELISA are the effective methods for detection of mouse Toxoplasmosis with high sensitivity,and PCR can detect Toxoplasma gondii DNA and diagnose toxolpasmosis with high specificity and sensitivity.