Objective To establish a route for administration of drugs to liver directly and reduce their influence on other organs. Methods A catheter was inserted in proper hepatic artery and remained one end of it outside under surgical operation microscope. Results The total number of rats in experiment was fifteen. Ten of them were alive and recovered from operation quickly. The reasons of death were vascular injury, liver damage, overdose of beparin and accident in anesthesia, which could be avoided by skilled operaters. Conclusion The above-mentioned method is simple, and easy to be mastered when drugs are given through the free end of the catheter.