Recent studies of Neisseria gonococci infection in mouse have yielded important progress in the pathogenesis of Neisseria gonococci. Estrogens have an anti-inflammatory effect. Estradiol-treated mouse may be infected from Neisseria gonococci. This Neisseria gonococci infection mouse model treated with estradiol may serve as a useful tool for ascertaining gonococcal pathogenesis, evaluating potential gonococcal vaccine candidates, screening and estimating bactericide.