Objective To investigate the effects of hypoxanthine and uricase inhibitor used singly or in combination on the concentration of serum uric acid in mice,to determine the optimal procedure,route and dosage of drug administration,and to evaluate the feasibility of this method to establish a mouse model of hyperuricemia.Methods After i.g.or i.p administration of hypoxanthine and i.p.or s.c.injection of uricase inhibitor,the concentration of serum uric acid was measured at different time intervals.Results At 1,3,5 hours after i.p.injection of hypoxanthine in a dose of 500 mg/kg and s.c.injection of uricase inhibitor in a dose of 50 mg/kg,the level of serum uric acid in mice was significantly increased(P<0.01).Moreover,positively reacting drugs can significantly reduce the increased serum uric acid in those mice(P<0.01).Conclusion Intraperitoneal injection of hypoxanthine in a dose of 500 mg/kg and subcutaneous injection of uricase inhibitor in a dose of 50 mg/kg,administered in an appropriate combination,is a reliable method to induce significant increase of serum uric acid in mice and can be used as a suitable method to establish a mouse model of hyperuricemia.