Objective To establish a simple and convenient method for detection of nine alleles in rabbits(such as Adh etc.).Metheods Isozyme electrophoresis and cellulose acetate membrane technique were used to monitor the alleles in rabbits.Results There were 5 loci polymorphism and significant difference among the population.Conclusion A survey of alleles distribution was made at 11 genetic loci among 4 closed rabbit colonies using frequently in Beijing area.So we can expand some alleles to keep the gene stability.