Objective To establish an platform to investigate embryos of KM mice. Methods The sperm corned from from transgenic mice of GFP fertilized and cuhuredin vitro with the ovum of KM mice getted by superovulation. The embryo was observed under fluorescence microscope. Results Sperm of GFP mice was no expression of green fluorescence in fluorescence microscope, while when fertilized with the KM females in vitro, the oocytes took on strong or weak expession of green fluorescent. Conclusion Strong or weak expression of green fluorescent could be the mark of embryos viability.