Objeetlve To find an economical and simple way to conduct cardiomyocyte culture. Methods Two methods, the 0.25% lysine-enzymatlc digestion and lissue pieces were designed for culturing cardiomyocytes from newborn rats. Results The 0.25% lysine-enzymatic digestion method provided sufficient amount of cells for research, but it took longer time and showed more pronounced damage to the cardiomyocytes. The tissue pieces method was easier to handle with less damage to the ceils, but the obtained amount of cardiomyocytes was limited. Conclusion Both two methods have their own advantages and disadvantages, and should be chosen depending on actual situation and demands.