Objective To establish normal values and ranges of blood biochemical measurements in Beagle dogs, to he used as reference values for long-term toxicity test. Methods Blood samples were taken from Beagle dogs in different age and sex. A number of blood biochemical parameters were measured with an automatic chemistry analyzer. Results Mean values and normal ranges of all data were obtained. Part of the biochemical values were influenced by sex difference. The activities of ALP and CK were weaker along with the increasing age of dogs. Conclusion In long-term toxicity test, it is necessary to pay attention to the influence of age and sex of Beagle dogs, when data obtained in an experiment are compared with their normal values and ranges.